PCR

• What does PCR stand for?
• Describe what PCR is in its general sense?
• Why do we amplify DNA in PCR?
• Get into some of the specifics of what the PCR technique helps do?
• Each reaction cycle causes the amount of DNA to increase how many times?
• In a standard PCR sequence (30 cycles) how many copies are created?
• PCR occurs in what type of laboratory apparatus is used?
• What are the three stages of PCR?
• What happens after the quantities of DNA have been created?
• Overview of the cycle

Gel Electrophoresis

• What is Gel electrophoresis in general terms?
• Explain in details how this works

DNA separation

• DNA may be cut into fragments using what?
• Different DNA samples will generate different what?
• Why do fragments separate
• What are DNA samples placed into what substance?
• How does hybridization probe work?